Year: 2018 | Month: February | Volume 11 | Issue 1
STMS Markers Proved Effective in Establishing Identity and Determining Genetic Purity in Commercial Seed Lots of ‘Pusa Rice Hybrid 10’
Abstract:
The identification of variety has attained paramount importance in the context of plant variety protection as well as to harness the maximum yield potential of any crop. Molecular fingerprinting is one of the most important tools to authenticate the identity and purity of seed lots of crop variety/hybrids. The present investigation was undertaken to establish the identity and genetic purity of 12 commercial hybrid lots of Pusa Rice Hybrid 10 (PRH 10) procured from different public and private seed companies including a farmer’s seed through molecular fingerprinting. Ten most informative sequence tagged microsatellite markers (STMS) were employed for fingerprinting of PRH 10. These markers together produced a unique fingerprint of ‘PRH 10’, which was found to be effective in the identification of this hybrid. Variation could not be observed among the twelve commercial seed lots obtained from different sources with respect to molecular profile of PRH 10. The results indicated the practical usefulness of STMS markers in the protection and identification of hybrids and their parental lines. However, plant-to-plant allelic variation was found within hybrid plants using markers RM 206 and RM 228. These two markers revealed the homozygous pattern in one plant of PRH 10. The occurrence of inter-plant variation at unlinked marker confirmed the molecular heterogeneity. This highlighted the importance of STMS markers in maintaining the genetic purity of the parental lines and hybrids which would be quite effective in preventing the fraudulent marketing of the popular hybrid/variety.
© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Print This Article Email This Article to Your Friend
